Monday, August 17, 2009

Progesterone Screening Tests

Ovarian Hormones

  1. estrogen
  2. estriol
  3. estradiol
  • Progesterone - A woman's progesterone levels are very high during pregnancy, along with estrogens, especially estriol.
  • Normal values of progesterone:
    During a normal menstrual cycle
    8 - 10 ng/ml
    During menopause (high levels)
    16 ng/ml

Visit this site to clarify confusions with estrogen and progesterone :

Screening Tests

Kober's Test

A modification of this test is known as Bachmann-Pettit test. Kober - for naturally occurring estrogens, based upon the production of a pink color (absorption maximum: 520 mmu) when an estrogen is heated in a mixture of phenol and sulfuric acid.

Kurzkor Ratner Test
Specimen:Urine (first morning urine)

  • ethyl acetate is the reagent that is used to extract the urine and after the purification, the extract is subjected to bioassay like in the Allen Doisy test.

Allen Doisy Test

  • Estrogenic Activity Test
  • the hormone to be tested is repeatedly injected to rats/mice.
  • (+) positive reaction is the dis appearance of WBC's in vaginal smear of the rats and the presence of cornified cells.

Precautions before taking the test:

  • note the last day of menstruation
  • Avoid taking of medications containing the hormone estrogen before taking the test(contraceptives)
  • Avoid strenuous activities
  • Progesterone and estrogen levels may fluctuate during the day

Increased Levels:

  • pregnancy
  • multiple pregnancy
  • H Mole
  • Cancer of the ovaries or adrenala glands
  • overproduction of hormones by the adrenal glands

Decreased Levels:

  • miscarriage
  • ovulation problems

References: Clinical Chemistry Principples,Procedures,Correlations (5th Edition) Michael Bishop,Edward Fody. Larry Schoeff

Images by:


  1. Edit your post as instructed beforehand. Cite references. See me on Monday.

  2. A mixture of Phenol and Sulfuric Acid thingie? Now those stuff are scary. Take care.

  3. I forgot to look for the normal values from the previous posts. hehehe. Good thing you mentioned it here.

    keep writing!


  4. Estrone is one of the three naturally occurring estrogens, the others being estradiol and estriol. Estrone is synthesized from androstenedione by the aromatase enzyme system in the ovaries and placenta, and is also synthesized from estradiol by 17-hydroxy steroid dehydrogenase in the liver. Estrone